NADH NAD Redox Index

Compare cellular NADH/NAD+ log10 ratio to a supplied reference.

Description

NADH/NAD redox-index screening reports the base-ten logarithm of a concentration ratio.

NADH/NAD redox-index screening reports the base-ten logarithm of a concentration ratio. The supplied comparison reference gives the flag its meaning.^[1]

Compare cellular NADH/NAD+ log10 ratio to a supplied reference.

When to use NADH NAD Redox Index

Use this tool to compare cellular NADH/NAD+ log10 ratio to a supplied reference.

Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.

How to use NADH NAD Redox Index and understand its results

Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.
Enter nadh, nad, referenceLog10Ratio using the field table and calculation rules, then run the tool.
Read log10RedoxRatio, aboveReference. The explanation below defines the units, signs, ordering and any flags or placeholder values.
If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.

Input field	Meaning	Value type; see rules below	Requirement
nadh	Nadh	number	Required
nad	Nad	number	Required
referenceLog10Ratio	Reference log 10 ratio	number	Required

Enter strictly positive compatible concentrations and a finite reference. Return the logarithmic index and inclusive comparison.

index=log10(NADH/NAD); flag=index≥reference

Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.

Output field	Meaning	Value type
log10RedoxRatio	Log 10 redox ratio	number
aboveReference	Above reference	boolean

json

{
  "input": {
    "nadh": 100,
    "nad": 10,
    "referenceLog10Ratio": 0.5
  },
  "output": {
    "log10RedoxRatio": 1,
    "aboveReference": true
  }
}

Limitations and assumptions

This is not a Nernst potential. Compartment, pH and enzyme equilibria are not modeled.

Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors. A successfully returned false flag is an evaluated outcome; its meaning is explained above.

Alternative or Complementary methods

Use Ketone Redox Ratio: Compute the β-hydroxybutyrate/acetoacetate redox proxy. For a complementary task, use Glutathione Redox Index: Screen glutathione redox ratio against a supplied log10 threshold.

References

NADH NAD Redox Index — tool.io

Similar or alternative tools

Ketone Redox Ratio
Compute the β-hydroxybutyrate/acetoacetate redox proxy.
Glutathione Redox Index
Screen glutathione redox ratio against a supplied log10 threshold.