Matrix Ion Suppression
Quantify matrix-induced ion suppression or enhancement using a post-extraction spike.
Description
Matrix-effect screening compares post-extraction spike response with a neat standard.
Matrix-effect screening compares post-extraction spike response with a neat standard. Values below or above equal response indicate suppression or enhancement under this arithmetic convention.1
Quantify matrix-induced ion suppression or enhancement using a post-extraction spike.
When to use Matrix Ion Suppression
Use this tool to quantify matrix-induced ion suppression or enhancement using a post-extraction spike.
Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.
How to use Matrix Ion Suppression and understand its results
- Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.
- Enter postExtractionSpikeArea, neatStandardArea using the field table and calculation rules, then run the tool.
- Read matrixEffectPercent, responsePercent. The explanation below defines the units, signs, ordering and any flags or placeholder values.
- If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.
| Input field | Meaning | Value type; see rules below | Requirement |
|---|---|---|---|
| postExtractionSpikeArea | Post extraction spike area | number | Required |
| neatStandardArea | Neat standard area | number | Required |
Enter nonnegative post-extraction area and positive neat-standard area. Return response and signed effect; negative effect indicates suppression and positive effect enhancement.
response%=100 postExtraction/neat; effect%=response%−100 Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.
| Output field | Meaning | Value type |
|---|---|---|
| matrixEffectPercent | Matrix effect percent | number |
| responsePercent | Response percent | number |
{
"input": {
"postExtractionSpikeArea": 80,
"neatStandardArea": 100
},
"output": {
"matrixEffectPercent": -20,
"responsePercent": 80
}
}Limitations and assumptions
Comparable spike amounts and acquisition conditions are assumed. Extraction recovery is excluded.
Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.
Alternative or Complementary methods
Use Process Efficiency: Measure total analytical process efficiency relative to a neat standard. For a complementary task, use Internal Standard Normalization: Normalize analyte peak areas against matched internal-standard injections.
References
-
Matrix Ion Suppression — tool.io
Similar or alternative tools
- Process Efficiency
Measure total analytical process efficiency relative to a neat standard.
- Internal Standard Normalization
Normalize analyte peak areas against matched internal-standard injections.