ECAR Glycolysis Screen
Screen extracellular acidification rate as a glycolysis proxy using a caller cutoff.
Description
An ECAR screen compares extracellular acidification rate with a caller-supplied minimum.
An ECAR screen compares extracellular acidification rate with a caller-supplied minimum. Its margin preserves the original rate units.1
Screen extracellular acidification rate as a glycolysis proxy using a caller cutoff.
When to use ECAR Glycolysis Screen
Use this tool to screen extracellular acidification rate as a glycolysis proxy using a caller cutoff.
Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.
How to use ECAR Glycolysis Screen and understand its results
- Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.
- Enter ecarMpHPerMin, minimumGlycolysisProxy using the field table and calculation rules, then run the tool.
- Read proxyAboveThreshold, thresholdMargin. The explanation below defines the units, signs, ordering and any flags or placeholder values.
- If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.
| Input field | Meaning | Value type; see rules below | Requirement |
|---|---|---|---|
| ecarMpHPerMin | Ecar mp h per min | number | Required |
| minimumGlycolysisProxy | Minimum glycolysis proxy | number | Required |
Enter nonnegative rate and minimum in mpH/min. Return signed margin and inclusive pass flag.
margin=ECAR−minimum; passes=margin≥0 Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.
| Output field | Meaning | Value type |
|---|---|---|
| proxyAboveThreshold | Proxy above threshold | boolean |
| thresholdMargin | Threshold margin | number |
{
"input": {
"ecarMpHPerMin": 40,
"minimumGlycolysisProxy": 30
},
"output": {
"proxyAboveThreshold": true,
"thresholdMargin": 10
}
}Limitations and assumptions
No glycolytic-flux conversion, buffering correction, cell normalization or physiological diagnosis is performed.
Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors. A successfully returned false flag is an evaluated outcome; its meaning is explained above.
Alternative or Complementary methods
Use Cell Normalized OCR: Normalize oxygen consumption to a caller-selected cell count. For a complementary task, use Lactate Glucose Yield: Compute apparent lactate yield per glucose and two-lactate carbon recovery.
References
-
ECAR Glycolysis Screen — tool.io
Similar or alternative tools
- Cell Normalized OCR
Normalize oxygen consumption to a caller-selected cell count.
- Lactate Glucose Yield
Compute apparent lactate yield per glucose and two-lactate carbon recovery.