HP1 Enrichment

Calculate HP1 enrichment after background correction.

Description

HP1 enrichment compares background-corrected IP and input signals.

HP1 enrichment compares background-corrected IP and input signals.^[1]

Calculate HP1 enrichment after background correction.

When to use HP1 Enrichment

Use this tool to calculate HP1 enrichment after background correction.

Prepare the already measured signals, counts, coordinates, fitted coefficients or sequence strings requested. Keep assay definitions, units, aligned lists and caller-selected thresholds consistent.

How to use HP1 Enrichment and understand its results

Prepare the already measured signals, counts, coordinates, fitted coefficients or sequence strings requested. Keep assay definitions, units, aligned lists and caller-selected thresholds consistent.
Enter hp1Signal, inputSignal, background using the field table and calculation rules, then run the tool.
Read correctedHp1, enrichmentFold. The explanation below defines the units, signs, ordering and any flags or placeholder values.
If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.

Input field	Meaning	Value type; see rules below	Requirement
hp1Signal	HP1 signal	number; minimum: 0	Required
inputSignal	Input signal	number; minimum: 0	Required
background	Background	number; minimum: 0	Required

Enter nonnegative signals/background; corrected input must be positive. Return corrected HP1 and fold.

corrected=max(0,signal−background); fold=correctedHP1/correctedInput

Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.

Output field	Meaning	Value type
correctedHp1	Corrected HP1 signal	number; minimum: 0
enrichmentFold	HP1 enrichment	number; minimum: 0

json

{
  "input": {
    "hp1Signal": 110,
    "inputSignal": 30,
    "background": 10
  },
  "output": {
    "correctedHp1": 100,
    "enrichmentFold": 5
  }
}

Limitations and assumptions

No antibody specificity, occupancy or significance is assessed.

Numeric inputs, where present, must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.

Alternative or Complementary methods

Use H2A.Z Enrichment: Calculate H2A.Z enrichment relative to H2A. For a complementary task, use MeDIP Enrichment: Calculate MeDIP enrichment after volume normalization.

References

HP1 Enrichment — tool.io

Similar or alternative tools

H2A.Z Enrichment
Calculate H2A.Z enrichment relative to H2A.
MeDIP Enrichment
Calculate MeDIP enrichment after volume normalization.