H2A.Z Enrichment

Calculate H2A.Z enrichment relative to H2A.

Description

H2A.Z enrichment is a ratio of supplied background-corrected signals.

H2A.Z enrichment is a ratio of supplied background-corrected signals.^[1]

Calculate H2A.Z enrichment relative to H2A.

When to use H2A.Z Enrichment

Use this tool to calculate H2A.Z enrichment relative to H2A.

Prepare the already measured signals, counts, coordinates, fitted coefficients or sequence strings requested. Keep assay definitions, units, aligned lists and caller-selected thresholds consistent.

How to use H2A.Z Enrichment and understand its results

Prepare the already measured signals, counts, coordinates, fitted coefficients or sequence strings requested. Keep assay definitions, units, aligned lists and caller-selected thresholds consistent.
Enter h2azSignal, h2aSignal, background using the field table and calculation rules, then run the tool.
Read correctedH2az, enrichmentFold. The explanation below defines the units, signs, ordering and any flags or placeholder values.
If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.

Input field	Meaning	Value type; see rules below	Requirement
h2azSignal	H2A.Z signal	number; minimum: 0	Required
h2aSignal	H2A signal	number; minimum: 0	Required
background	Background	number; minimum: 0	Required

Enter nonnegative signals/background with positive corrected input; return corrected H2A.Z and fold.

fold=max(0,H2AZ−background)/max(0,input−background)

Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.

Output field	Meaning	Value type
correctedH2az	Corrected H2A.Z	number; minimum: 0
enrichmentFold	Exchange enrichment	number; minimum: 0

json

{
  "input": {
    "h2azSignal": 110,
    "h2aSignal": 30,
    "background": 10
  },
  "output": {
    "correctedH2az": 100,
    "enrichmentFold": 5
  }
}

Limitations and assumptions

No occupancy, antibody specificity or regulatory function is established.

Numeric inputs, where present, must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.

Alternative or Complementary methods

Use HP1 Enrichment: Calculate HP1 enrichment after background correction. For a complementary task, use Bivalent Promoter: Flag promoter bivalency from activating and repressive mark signals.

References

H2A.Z Enrichment — tool.io

Similar or alternative tools

HP1 Enrichment
Calculate HP1 enrichment after background correction.
Bivalent Promoter
Flag promoter bivalency from activating and repressive mark signals.