Description
Apply a supplied fluorescence inner-filter approximation.
Laboratory quantities depend on sample volume, concentration basis, blank correction, geometry and calibration. A dilution, recovery fraction or signal correction evaluates a supplied measurement model without validating the assay, identifying a substance or establishing biological or clinical performance.
Apply a supplied fluorescence inner-filter approximation.
When to use this tool
Use Fluorescence Assay Innerfilter One Wavelength Term when checking the stated laboratory bookkeeping, signal, sample or measurement convention.
How to use the tool and how it works
- Enter observed, excitationAbsorbance, emissionAbsorbance. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read corrected, factor. Use the output convention below.
Apply a supplied fluorescence inner-filter approximation. factor = 10^((excitationAbsorbance+emissionAbsorbance)/2), corrected = observed*factor.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| observed | Observed | Required. Finite number. |
| excitationAbsorbance | Excitation Absorbance | Required. Finite number. |
| emissionAbsorbance | Emission Absorbance | Required. Finite number. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| corrected | Corrected | Required. Finite number. |
| factor | 10^((excitationAbsorbance+emissionAbsorbance)/2), under the stated inner-filter approximation. | Required. Finite number. |
{
"input": {
"observed": 100,
"excitationAbsorbance": 0.2,
"emissionAbsorbance": 0.2
},
"output": {
"corrected": 158.48931924611136,
"factor": 1.5848931924611136
}
}Limitations and assumptions
- Absorbances use decadic convention; this local model does not verify optical geometry, concentration range or actual assay validity.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use verified sample records, calibrated equipment and an appropriate validated measurement method; assess uncertainty and practical laboratory conditions independently.
References
Similar or alternative tools
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