Description
Interpolate a chromatogram baseline at a peak time.
Laboratory quantities depend on sample volume, concentration basis, blank correction, geometry and calibration. A dilution, recovery fraction or signal correction evaluates a supplied measurement model without validating the assay, identifying a substance or establishing biological or clinical performance.
Interpolate a chromatogram baseline at a peak time.
When to use this tool
Use Chromatogram Peak Baseline Slope when checking the stated laboratory bookkeeping, signal, sample or measurement convention.
How to use the tool and how it works
- Enter startTime, endTime, startBaseline, endBaseline, peakTime, observedHeight. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read slope, baselineAtPeak, correctedHeight. Use the output convention below.
Interpolate a chromatogram baseline at a peak time. Require endTime > startTime and peakTime within the closed interval; slope = baseline difference/time difference, baselineAtPeak interpolates linearly, correctedHeight subtracts it from observedHeight.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| startTime | Start Time | Required. Finite number. |
| endTime | End Time | Required. Finite number. |
| startBaseline | Start Baseline | Required. Finite number. |
| endBaseline | End Baseline | Required. Finite number. |
| peakTime | Peak Time | Required. Finite number. |
| observedHeight | Observed Height | Required. Finite number. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| slope | Slope | Required. Finite number. |
| baselineAtPeak | Baseline At Peak | Required. Finite number. |
| correctedHeight | Corrected Height | Required. Finite number. |
{
"input": {
"startTime": 0,
"endTime": 10,
"startBaseline": 2,
"endBaseline": 4,
"peakTime": 5,
"observedHeight": 12
},
"output": {
"slope": 0.2,
"baselineAtPeak": 3,
"correctedHeight": 9
}
}Limitations and assumptions
- This is one supplied baseline segment rather than peak detection, area integration or assay validation.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use verified sample records, calibrated equipment and an appropriate validated measurement method; assess uncertainty and practical laboratory conditions independently.
References
Similar or alternative tools
- Biosensor Baseline Drift One Interval
Return a biosensor signal after linear baseline drift removal.
- Fluorescence Assay Innerfilter One Wavelength Term
Apply a supplied fluorescence inner-filter approximation.