Description
Return blank-subtracted photon rate and reference gap.
Fluorescence signals combine excitation, emission, detector response and background. An intensity ratio, effective lifetime or anisotropy correction describes a supplied optical model; measured signal fractions and ideal energy-transfer efficiencies are different quantities and need appropriate calibration.
Return blank-subtracted photon rate and reference gap.
When to use this tool
Use Fluorescence Photon Exposure Gap when checking the stated fluorescence, lifetime, transfer or polarization correction.
How to use the tool and how it works
- Enter signalPhotons, backgroundPhotons, exposureDuration, referenceRate. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read netPhotons, correctedRate, rateGap. Use the output convention below.
Return blank-subtracted photon rate and reference gap. Require nonnegative photon inputs and exposureDuration > 0; netPhotons = signal-background, correctedRate = net/exposure, rateGap subtracts referenceRate.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| signalPhotons | Signal Photons | Required. Finite number; minimum 0. |
| backgroundPhotons | Background Photons | Required. Finite number; minimum 0. |
| exposureDuration | Exposure Duration | Required. Finite number; strictly above 0. |
| referenceRate | Reference Rate | Required. Finite number. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| netPhotons | Net Photons | Required. Finite number. |
| correctedRate | Corrected Rate | Required. Finite number. |
| rateGap | Rate Gap | Required. Finite number. |
{
"input": {
"signalPhotons": 100,
"backgroundPhotons": 20,
"exposureDuration": 4,
"referenceRate": 15
},
"output": {
"netPhotons": 80,
"correctedRate": 20,
"rateGap": 5
}
}Limitations and assumptions
- Inputs may be noninteger counts; negative corrected signals remain numerical results rather than physical negative photon emission.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use calibrated optical measurements and a complete suitable photophysical model, including background, cross-talk and detector effects, for molecular or kinetic inference.
References
Similar or alternative tools
- Fluorescence Lifetime Radiative Rate Factor
Return effective fluorescence lifetime and yield from rates.
- Fluorescence Donor Leakage Term
Subtract supplied donor leakage from acceptor signal.