Description
Return G-corrected fluorescence anisotropy with a denominator flag.
Fluorescence signals combine excitation, emission, detector response and background. An intensity ratio, effective lifetime or anisotropy correction describes a supplied optical model; measured signal fractions and ideal energy-transfer efficiencies are different quantities and need appropriate calibration.
Return G-corrected fluorescence anisotropy with a denominator flag.
When to use this tool
Use Fluorescence G Factor Domain Gap when checking the stated fluorescence, lifetime, transfer or polarization correction.
How to use the tool and how it works
- Enter parallel, perpendicular, suppliedG. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read denominator, defined, anisotropy. Inspect defined before interpreting the associated values.
Return G-corrected fluorescence anisotropy with a denominator flag. Require nonnegative parallel/perpendicular intensities and G > 0; denominator = parallel+2*G*perpendicular, anisotropy = (parallel-G*perpendicular)/denominator, or zero with defined false.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| parallel | Parallel | Required. Finite number; minimum 0. |
| perpendicular | Perpendicular | Required. Finite number; minimum 0. |
| suppliedG | Supplied G | Required. Finite number; strictly above 0. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| denominator | Denominator | Required. Finite number. |
| defined | Whether total G-corrected intensity is nonzero; zero anisotropy is a placeholder when false. | Required. Boolean. |
| anisotropy | Anisotropy | Required. Finite number. |
{
"input": {
"parallel": 4,
"perpendicular": 1,
"suppliedG": 1
},
"output": {
"denominator": 6,
"defined": true,
"anisotropy": 0.5
}
}Limitations and assumptions
- The G factor is supplied, and zero total signal produces a placeholder rather than measured zero anisotropy.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use calibrated optical measurements and a complete suitable photophysical model, including background, cross-talk and detector effects, for molecular or kinetic inference.
References
Similar or alternative tools
- Fluorescence Anisotropy G Factor Sensitivity
Return anisotropy and sensitivity to the G factor.
- Fluorescence Photon Exposure Gap Tool
Calculate fluorescence photon exposure gap from explicit caller-supplied inputs.