Description
Return anisotropy and sensitivity to the G factor.
Fluorescence signals combine excitation, emission, detector response and background. An intensity ratio, effective lifetime or anisotropy correction describes a supplied optical model; measured signal fractions and ideal energy-transfer efficiencies are different quantities and need appropriate calibration.
Return anisotropy and sensitivity to the G factor.
When to use this tool
Use Fluorescence Anisotropy G Factor Sensitivity when checking the stated fluorescence, lifetime, transfer or polarization correction.
How to use the tool and how it works
- Enter parallelIntensity, perpendicularIntensity, gFactor. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read anisotropy, derivative. Use the output convention below.
Return anisotropy and sensitivity to the G factor. Require nonnegative intensities, positive G and positive D = parallel+2*G*perpendicular; anisotropy = (parallel-G*perpendicular)/D, derivative = -3*parallel*perpendicular/D².1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| parallelIntensity | Parallel Intensity | Required. Finite number; minimum 0. |
| perpendicularIntensity | Perpendicular Intensity | Required. Finite number; minimum 0. |
| gFactor | G Factor | Required. Finite number; strictly above 0. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| anisotropy | Anisotropy | Required. Finite number. |
| derivative | Partial derivative of anisotropy with respect to G, holding both intensities fixed. | Required. Finite number. |
{
"input": {
"parallelIntensity": 100,
"perpendicularIntensity": 50,
"gFactor": 1
},
"output": {
"anisotropy": 0.25,
"derivative": -0.375
}
}Limitations and assumptions
- The derivative treats intensities fixed; it is not a full measurement uncertainty or independent calibration of G.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use calibrated optical measurements and a complete suitable photophysical model, including background, cross-talk and detector effects, for molecular or kinetic inference.
References
Similar or alternative tools
- Fluorescence G Factor Domain Gap
Return G-corrected fluorescence anisotropy with a denominator flag.
- Fluorescence Lifetime Radiative Rate Factor Tool
Calculate fluorescence lifetime radiative rate factor from explicit caller-supplied inputs.