Use RNA Conc From A260 when you need to convert an A260 absorbance reading into RNA concentration using the 40 ng/µL per absorbance unit factor. Enter Absorbance A260 and Dilution factor using the definitions and units below so the result can be reproduced.

Absorbance A260

A finite measured or assumed value greater than or equal to 0.

Dilution factor

A finite measured or assumed value greater than or equal to 1.

The published tool contract uses this rule: Convert an A260 absorbance reading into RNA concentration using the 40 ng/µL per absorbance unit factor.

RNA concentration

The calculated rna concentration in ng/µL.

A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.

Retain the original Absorbance A260 and Dilution factor values and their units alongside the result. DNA Conc From A260 is useful when you instead need to convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor. Protein Conc From A280 provides another view by helping you convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).

• DNA Conc From A260

  Convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor.

• Protein Conc From A280

  Convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).

• Bradford Protein Conc

  Convert a Bradford assay A595 reading into protein concentration using the slope of the standard curve.