DNA Conc From A260
Convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor.
Description
Convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor.
DNA Conc From A260 is a focused biology tool that convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use DNA Conc From A260
Use DNA Conc From A260 when you need to convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor. Enter Absorbance A260 and Dilution factor using the definitions and units below so the result can be reproduced.
- Absorbance A260
- A finite measured or assumed value greater than or equal to 0.
- Dilution factor
- A finite measured or assumed value greater than or equal to 1.
How DNA Conc From A260 calculates the result
The published tool contract uses this rule: Convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor.
- DNA concentration
- The calculated dna concentration in ng/µL.
Limitations of DNA Conc From A260
A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.
Alternative or Complementary measurements
Retain the original Absorbance A260 and Dilution factor values and their units alongside the result. RNA Conc From A260 is useful when you instead need to convert an A260 absorbance reading into RNA concentration using the 40 ng/µL per absorbance unit factor. Protein Conc From A280 provides another view by helping you convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).
References
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Nucleic acid quantitation — Wikipedia contributors
Similar or alternative tools
- RNA Conc From A260
Convert an A260 absorbance reading into RNA concentration using the 40 ng/µL per absorbance unit factor.
- Protein Conc From A280
Convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).
- Primer Concentration nM
Convert a single-stranded DNA primer mass concentration in ng per microlitre to nanomolar using an approximate average nucleotide mass of 330 grams per mole.