Use this conversion after fitting a Bradford standard curve under the same reagent, plate, wavelength, blank, incubation, and sample-dilution conditions as the unknown.

Absorbance A595

A finite measured or assumed value greater than or equal to 0.

Calibration slope

A finite measured or assumed value.

The implementation follows this calculation rule: Convert a Bradford assay A595 reading into protein concentration using the slope of the standard curve.^[1]

Protein concentration

The calculated protein concentration in µg/mL.

The response depends on protein composition and is not linear over every concentration. Detergents, buffers, precipitates, saturation, an uncorrected blank, or using a standard unlike the sample protein can bias the concentration. Ratios and percentages become unstable when their denominator is small, uncertain, or defined differently between samples.

Inspect the full standard curve and diluted replicates rather than relying on one absorbance. A BCA, Lowry, UV-absorbance, or amino-acid analysis may be more appropriate for incompatible buffers or higher accuracy.

• Lowry Protein Conc

  Convert a Lowry assay A750 reading into protein concentration by subtracting the blank intercept and dividing by the standard curve slope.

• Protein Conc From A280

  Convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).

• DNA Conc From A260

  Convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor.