Protein Conc From A280
Convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).
Description
Convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).
Protein Conc From A280 is a focused biology tool that convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm). It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use Protein Conc From A280
Use Protein Conc From A280 when you need to convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm). Enter Absorbance A280, Dilution factor, Extinction coefficient, and Optical path length using the definitions and units below so the result can be reproduced.
- Absorbance A280
- A finite measured or assumed value greater than or equal to 0.
- Dilution factor
- A finite measured or assumed value greater than or equal to 1.
- Extinction coefficient
- A finite measured or assumed value, expressed in mL/(mg·cm).
- Optical path length
- A finite measured or assumed value greater than or equal to 5e-324, expressed in cm.
How Protein Conc From A280 calculates the result
The published tool contract uses this rule: Convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).1
- Protein concentration
- The calculated protein concentration in mg/mL.
Limitations of Protein Conc From A280
A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.
Alternative or Complementary measurements
Retain the original Absorbance A280, Dilution factor, Extinction coefficient, and Optical path length values and their units alongside the result. Bradford Protein Conc is useful when you instead need to convert a Bradford assay A595 reading into protein concentration using the slope of the standard curve. DNA Conc From A260 provides another view by helping you convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor.
References
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Ultraviolet–visible spectroscopy — Wikipedia contributors
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