Lowry Protein Conc
Convert a Lowry assay A750 reading into protein concentration by subtracting the blank intercept and dividing by the standard curve slope.
Description
Convert a Lowry assay A750 reading into protein concentration by subtracting the blank intercept and dividing by the standard curve slope.
Lowry Protein Conc is a focused biology tool that convert a Lowry assay A750 reading into protein concentration by subtracting the blank intercept and dividing by the standard curve slope. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use Lowry Protein Conc
Use Lowry Protein Conc when you need to convert a Lowry assay A750 reading into protein concentration by subtracting the blank intercept and dividing by the standard curve slope. Enter Absorbance A750, Calibration intercept, and Calibration slope using the definitions and units below so the result can be reproduced.
- Absorbance A750
- A finite measured or assumed value greater than or equal to 0.
- Calibration intercept
- A finite measured or assumed value.
- Calibration slope
- A finite measured or assumed value.
How Lowry Protein Conc calculates the result
The published tool contract uses this rule: Convert a Lowry assay A750 reading into protein concentration by subtracting the blank intercept and dividing by the standard curve slope.1
- Protein concentration
- The calculated protein concentration in µg/mL.
Limitations of Lowry Protein Conc
A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.
Alternative or Complementary measurements
Retain the original Absorbance A750, Calibration intercept, and Calibration slope values and their units alongside the result. Bradford Protein Conc is useful when you instead need to convert a Bradford assay A595 reading into protein concentration using the slope of the standard curve. Protein Conc From A280 provides another view by helping you convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).
References
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Lowry protein assay — Wikipedia contributors
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