Use Primer Concentration nM when you need to convert a single-stranded DNA primer mass concentration in ng per microlitre to nanomolar using an approximate average nucleotide mass of 330 grams per mole. Enter Concentration ng/µL and Length nt using the definitions and units below so the result can be reproduced.

Concentration ng/µL

A finite measured or assumed value.

Length nt

An integer count or setting greater than or equal to 1.

The published tool contract uses this rule: Convert a single-stranded DNA primer mass concentration in ng per microlitre to nanomolar using an approximate average nucleotide mass of 330 grams per mole.

Primer concentration nM

The calculated primer concentration nm in nM.

A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.

Retain the original Concentration ng/µL and Length nt values and their units alongside the result. DNA Conc From A260 is useful when you instead need to convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor. Protein Conc From A280 provides another view by helping you convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).

• DNA Conc From A260

  Convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor.

• Protein Conc From A280

  Convert an A280 absorbance reading into protein concentration via the Beer-Lambert mass extinction coefficient in mL/(mg·cm).

• RNA Conc From A260

  Convert an A260 absorbance reading into RNA concentration using the 40 ng/µL per absorbance unit factor.