Description
Calculate a supplied electrophoretic relative mobility.
Biochemical quantities depend on species definitions, mass versus amount basis, reference states, blank models and assay conditions. A free/bound difference does not establish equilibrium, a per-volume activity differs from activity per protein mass, and a dilution factor differs from its reciprocal aliquot fraction.
Calculate a supplied electrophoretic relative mobility.
When to use this tool
Use Electrophoresis Relative Mobility when you already have analyteDistance, referenceFrontDistance and need to calculate a supplied electrophoretic relative mobility.
How to use the tool and how it works
- Enter analyteDistance, referenceFrontDistance. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read relativeMobility. Use the output convention below.
Require referenceFrontDistance>0; divide signed analyteDistance by that distance.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| analyteDistance | Analyte Distance | Required. Finite number. |
| referenceFrontDistance | Reference Front Distance | Required. Finite number; strictly above 0. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| relativeMobility | Relative Mobility | Required. Finite number. |
{
"input": {
"analyteDistance": 32,
"referenceFrontDistance": 40
},
"output": {
"relativeMobility": 0.8
}
}Limitations and assumptions
- The origin and distance unit must match. Negative or above-one values are accepted; no band detection, molecular size, identity or run quality is inferred.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use the full assay, reaction and calibration method with verified sample records. Check units, reference definitions, blanks and experimental assumptions before interpreting a local product, fraction or rate as a valid biochemical measurement.
References
Similar or alternative tools
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