Description
Subtract supplied chromatography void time from retention time.
Biochemical quantities depend on species definitions, mass versus amount basis, reference states, blank models and assay conditions. A free/bound difference does not establish equilibrium, a per-volume activity differs from activity per protein mass, and a dilution factor differs from its reciprocal aliquot fraction.
Subtract supplied chromatography void time from retention time.
When to use this tool
Use Chromatography Void Time Corrected Delay when you already have voidTime, retentionTime and need to subtract supplied chromatography void time from retention time.
How to use the tool and how it works
- Enter voidTime, retentionTime. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read signedGap. Use the output convention below.
Return retentionTime-voidTime with signs preserved.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| voidTime | Void Time | Required. Finite number. |
| retentionTime | Retention Time | Required. Finite number. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| signedGap | Signed Gap | Required. Finite number. |
{
"input": {
"voidTime": 12,
"retentionTime": 17
},
"output": {
"signedGap": 5
}
}Limitations and assumptions
- Both times require matching units and origins. No peak, void marker, corrected retention factor, column efficiency or compound identity is established.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use the full assay, reaction and calibration method with verified sample records. Check units, reference definitions, blanks and experimental assumptions before interpreting a local product, fraction or rate as a valid biochemical measurement.
References
Similar or alternative tools
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