Semiconductor Bio Fet Sensitivity Calculator
Extract actual BioFET response versus log10 independently assayed analyte concentration, signed slope and fit evidence; not diagnostic sensitivity/specificity, analyte identity or clinical detection.
Description
BioFET calibration can express voltage response per decade of independently assayed concentration.
BioFET calibration can express voltage response per decade of independently assayed concentration.1
This instrumental sensitivity differs from diagnostic sensitivity and specificity.
When to use Semiconductor Bio Fet Sensitivity Calculator
Use this tool when you need to calculate the stated measurement summary or conditional model from matched observations. Prepare independently matched device or specimen measurements, units, branch selection, and model or calibration conventions.
How to use Semiconductor Bio Fet Sensitivity Calculator and understand its results
- Prepare independently matched device or specimen measurements, units, branch selection, and model or calibration conventions.
- Enter observedBioAnalyteConcentrations, observedBioFetResponsesV, following the field table and calculation rules below, then run the tool.
- Read measuredBioFetVoltsPerDecade, responseInterceptV, bioFitRSquared. Use the output table and calculation rules below to interpret units, signs, crossings, and flags.
- Correct any input issues and run again. Before using a successful result, review its units, signs, evidence coverage, and stated model or reference assumptions.
| Input field | Meaning | Value type | Requirement |
|---|---|---|---|
| observedBioAnalyteConcentrations | Observed bio analyte concentrations | list of number | Required |
| observedBioFetResponsesV | Observed bio fet responses v | list of number | Required |
Require positive concentrations and aligned finite voltage responses. Fit response versus log10(concentration) with a free intercept, returning its signed slope, voltage intercept, and R². The fitted slope may be zero or negative.
The regression helper accepts 2–10000 aligned finite pairs with nonzero predictor variance. It fits ordinary least squares with a free intercept using shifted coordinates; samples need not be sorted unless this tool additionally requires it. R² is clamped to [0,1] and is one for constant responses. Transformed fits use the stated log or reciprocal predictor/response basis; R² is a fit summary rather than a confidence interval.
V=a+b×log10(concentration) Read these symbols with the field meanings and branch or reference rules above.
| Output field | Meaning | Value type |
|---|---|---|
| measuredBioFetVoltsPerDecade | Measured bio fet volts per decade | number |
| responseInterceptV | Response intercept v | number |
| bioFitRSquared | Bio fit r squared | number |
{
"input": {
"observedBioAnalyteConcentrations": [
1,
10,
100
],
"observedBioFetResponsesV": [
1,
2,
3
]
},
"output": {
"measuredBioFetVoltsPerDecade": 1,
"responseInterceptV": 1,
"bioFitRSquared": 1
}
}Limitations and assumptions
Measurements, branch identification, units, and model applicability must be independently established. Results do not supply universal material constants, a complete physical model, or operating-condition approval.
Missing required fields, unknown fields, wrong types, and violated calculation rules return input issues. Numeric values must be finite; nonfinite numeric outputs are rejected. Arrays must be dense, with no missing cells. Numeric lists allow at most 10000 entries, subject to smaller tool-specific bounds above.
Alternative or Complementary methods
For a related task, use Semiconductor Isfet Ph Sensitivity Calculator: ISFET pH response is a measured voltage slope on an independently matched calibration. A temperature-dependent Nernst magnitude gives a separate comparison scale whose sign is not imposed on that measured response. For a complementary step, use Semiconductor MESFET Transconductance Calculator: Transconductance is the local rate of drain-current change with gate voltage on a matched transistor branch. A measured MESFET trace supplies that slope without assuming a saturation-current parameter model.
References
Similar or alternative tools
- Semiconductor Isfet Ph Sensitivity Calculator
ISFET pH response is a measured voltage slope on an independently matched calibration. A temperature-dependent Nernst magnitude gives a separate comparison scale whose sign is not imposed on that measured response.
- Semiconductor MESFET Transconductance Calculator
Transconductance is the local rate of drain-current change with gate voltage on a matched transistor branch. A measured MESFET trace supplies that slope without assuming a saturation-current parameter model.