Proton Leak Fraction
Measure oligomycin-resistant proton leak after non-mitochondrial subtraction.
Description
Proton-leak fraction compares oligomycin-resistant OCR with corrected basal OCR.
Proton-leak fraction compares oligomycin-resistant OCR with corrected basal OCR. Non-mitochondrial subtraction is applied to both relevant rates.1
Measure oligomycin-resistant proton leak after non-mitochondrial subtraction.
When to use Proton Leak Fraction
Use this tool to measure oligomycin-resistant proton leak after non-mitochondrial subtraction.
Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.
How to use Proton Leak Fraction and understand its results
- Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.
- Enter oligomycinOcr, nonMitochondrialOcr, basalOcr using the field table and calculation rules, then run the tool.
- Read protonLeakOcr, protonLeakPercent. The explanation below defines the units, signs, ordering and any flags or placeholder values.
- If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.
| Input field | Meaning | Value type; see rules below | Requirement |
|---|---|---|---|
| oligomycinOcr | Oligomycin ocr | number | Required |
| nonMitochondrialOcr | Non mitochondrial ocr | number | Required |
| basalOcr | Basal ocr | number | Required |
Enter finite rates with nonMito≤oligomycin≤basal and basal strictly above nonMito. Return corrected leak OCR and its percentage.
leak=oligomycin−nonMito; leakPercent=100 leak/(basal−nonMito) Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.
| Output field | Meaning | Value type |
|---|---|---|
| protonLeakOcr | Proton leak ocr | number |
| protonLeakPercent | Proton leak percent | number |
{
"input": {
"oligomycinOcr": 30,
"nonMitochondrialOcr": 10,
"basalOcr": 110
},
"output": {
"protonLeakOcr": 20,
"protonLeakPercent": 20
}
}Limitations and assumptions
No assay processing, inhibitor validation or mechanistic leakage measurement is performed. The rates must describe a compatible assay basis.
Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.
Alternative or Complementary methods
Use ATP Coupling Efficiency: Calculate ATP-linked oxygen consumption and mitochondrial coupling efficiency. For a complementary task, use Spare Respiratory Capacity: Measure spare respiratory capacity relative to corrected basal OCR.
References
-
Proton Leak Fraction — tool.io
Similar or alternative tools
- ATP Coupling Efficiency
Calculate ATP-linked oxygen consumption and mitochondrial coupling efficiency.
- Spare Respiratory Capacity
Measure spare respiratory capacity relative to corrected basal OCR.