ATP Coupling Efficiency
Calculate ATP-linked oxygen consumption and mitochondrial coupling efficiency.
Description
ATP-coupling efficiency relates oligomycin-sensitive OCR to corrected basal respiration.
ATP-coupling efficiency relates oligomycin-sensitive OCR to corrected basal respiration. Both rates share the supplied non-mitochondrial reference.1
Calculate ATP-linked oxygen consumption and mitochondrial coupling efficiency.
When to use ATP Coupling Efficiency
Use this tool to calculate ATP-linked oxygen consumption and mitochondrial coupling efficiency.
Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.
How to use ATP Coupling Efficiency and understand its results
- Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.
- Enter basalOcr, oligomycinOcr, nonMitoOcr using the field table and calculation rules, then run the tool.
- Read atpLinkedOcr, couplingEfficiencyPercent. The explanation below defines the units, signs, ordering and any flags or placeholder values.
- If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.
| Input field | Meaning | Value type; see rules below | Requirement |
|---|---|---|---|
| basalOcr | Basal ocr | number | Required |
| oligomycinOcr | Oligomycin ocr | number | Required |
| nonMitoOcr | Non mito ocr | number | Required |
Enter finite rates with nonMito≤oligomycin≤basal and basal>nonMito. Return ATP-linked OCR and percentage.
ATP-linked=basal−oligomycin; efficiency%=100 ATP-linked/(basal−nonMito) Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.
| Output field | Meaning | Value type |
|---|---|---|
| atpLinkedOcr | Atp linked ocr | number |
| couplingEfficiencyPercent | Coupling efficiency percent | number |
{
"input": {
"basalOcr": 100,
"oligomycinOcr": 40,
"nonMitoOcr": 20
},
"output": {
"atpLinkedOcr": 60,
"couplingEfficiencyPercent": 75
}
}Limitations and assumptions
Absolute rates can be signed if ordering holds. No assay processing or ATP amount, yield or turnover is measured.
Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.
Alternative or Complementary methods
Use Proton Leak Fraction: Measure oligomycin-resistant proton leak after non-mitochondrial subtraction. For a complementary task, use Spare Respiratory Capacity: Measure spare respiratory capacity relative to corrected basal OCR.
References
-
ATP Coupling Efficiency — tool.io
Similar or alternative tools
- Proton Leak Fraction
Measure oligomycin-resistant proton leak after non-mitochondrial subtraction.
- Spare Respiratory Capacity
Measure spare respiratory capacity relative to corrected basal OCR.