Adenylate Kinase Quotient

Check adenylate kinase mass-action quotient against an injected equilibrium value.

Description

An adenylate-kinase quotient screen compares a supplied nucleotide ratio with a chosen target.

An adenylate-kinase quotient screen compares a supplied nucleotide ratio with a chosen target. The formula fixes the reaction-quotient direction used by this tool.^[1]

Check adenylate kinase mass-action quotient against an injected equilibrium value.

When to use Adenylate Kinase Quotient

Use this tool to check adenylate kinase mass-action quotient against an injected equilibrium value.

Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.

How to use Adenylate Kinase Quotient and understand its results

Prepare the measured profile, formula counts, upstream model outputs or caller-defined reference criteria requested. Keep paired entries aligned and units, sample basis and preprocessing consistent.
Enter adp, atp, amp, targetQuotient, tolerance using the field table and calculation rules, then run the tool.
Read massActionQuotient, equilibriumMatch. The explanation below defines the units, signs, ordering and any flags or placeholder values.
If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.

Input field	Meaning	Value type; see rules below	Requirement
adp	Adp	number	Required
atp	Atp	number	Required
amp	Amp	number	Required
targetQuotient	Target quotient	number	Required
tolerance	Tolerance	number	Required

Enter positive ATP/AMP, nonnegative ADP, target and tolerance. Return quotient and inclusive target-window flag.

quotient=ADP²/(ATP×AMP); passes=|quotient−target|≤tolerance

Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.

Output field	Meaning	Value type
massActionQuotient	Mass action quotient	number
equilibriumMatch	Equilibrium match	boolean

json

{
  "input": {
    "adp": 2,
    "atp": 2,
    "amp": 2,
    "targetQuotient": 1,
    "tolerance": 0
  },
  "output": {
    "massActionQuotient": 1,
    "equilibriumMatch": true
  }
}

Limitations and assumptions

Activities, equilibrium constant, magnesium binding and reaction-direction conventions are not resolved automatically.

Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors. A successfully returned false flag is an evaluated outcome; its meaning is explained above.

Alternative or Complementary methods

Use Adenylate Energy Charge: Calculate adenylate energy charge from ATP, ADP, and AMP. For a complementary task, use Acetylcoa Coa Ratio: Calculate acetyl-CoA to free-CoA abundance ratio and acylated share.

References

Adenylate Kinase Quotient — tool.io

Similar or alternative tools

Adenylate Energy Charge
Calculate adenylate energy charge from ATP, ADP, and AMP.
Acetylcoa Coa Ratio
Calculate acetyl-CoA to free-CoA abundance ratio and acylated share.