Optogenetic Pulse Dose
Calculate pulsed optogenetic duty cycle, delivered energy, and radiant dose.
Description
Pulsed optical dose combines pulse-width duty cycle with an illumination power.
Pulsed optical dose combines pulse-width duty cycle with an illumination power. The implementation applies duty cycle to the field named averagePowerMilliwatts.1
Calculate pulsed optogenetic duty cycle, delivered energy, and radiant dose.
When to use Optogenetic Pulse Dose
Use this tool to calculate pulsed optogenetic duty cycle, delivered energy, and radiant dose.
Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
How to use Optogenetic Pulse Dose and understand its results
- Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
- Enter averagePowerMilliwatts, pulseWidthMilliseconds, pulseRateHertz, exposureSeconds, illuminatedAreaMm2 using the field table and calculation rules, then run the tool.
- Read dutyCycle, deliveredEnergyJoules, radiantDoseJPerCm2. The explanation below defines the units, signs, ordering and any flags or placeholder values.
- If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.
| Input field | Meaning | Value type; see rules below | Requirement |
|---|---|---|---|
| averagePowerMilliwatts | Average optical power (mW) | number; minimum: 0 | Required |
| pulseWidthMilliseconds | Pulse width (ms) | number; minimum: 0 | Required |
| pulseRateHertz | Pulse rate (Hz) | number; minimum: 0 | Required |
| exposureSeconds | Exposure duration (s) | number; minimum: 0 | Required |
| illuminatedAreaMm2 | Illuminated area (mm²) | number; minimum: 0 | Required |
Enter nonnegative power, width, rate and exposure and positive area. Return duty, joules and J/cm². For this model, supply an on-pulse power basis; an already time-averaged power would be scaled twice.
duty=width_ms×rate/1000; energy=power_mW/1000×duty×time; dose=energy/(area_mm²/100) Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.
| Output field | Meaning | Value type |
|---|---|---|
| dutyCycle | Pulse duty cycle | number; minimum: 0 |
| deliveredEnergyJoules | Delivered optical energy (J) | number; minimum: 0 |
| radiantDoseJPerCm2 | Radiant dose (J/cm²) | number; minimum: 0 |
{
"input": {
"averagePowerMilliwatts": 10,
"pulseWidthMilliseconds": 10,
"pulseRateHertz": 10,
"exposureSeconds": 10,
"illuminatedAreaMm2": 1
},
"output": {
"dutyCycle": 0.1,
"deliveredEnergyJoules": 0.01,
"radiantDoseJPerCm2": 1
}
}Limitations and assumptions
Duty is not capped at one. No opsin response, tissue attenuation, treatment efficacy or safety criterion is supplied.
Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.
Alternative or Complementary methods
Use Q-Switch Peak Power: Calculate triangular-pulse peak power and pulse-train average power. For a complementary task, use Photobiomodulation Dose: Calculate delivered energy and fluence for photobiomodulation.
References
-
Optogenetic Pulse Dose — tool.io
Similar or alternative tools
- Q-Switch Peak Power
Calculate triangular-pulse peak power and pulse-train average power.
- Photobiomodulation Dose
Calculate delivered energy and fluence for photobiomodulation.