Microdissection Pulse Overlap
Calculate pulse spacing and linear overlap for laser microdissection.
Description
Microdissection pulse overlap compares scan spacing with spot diameter.
Microdissection pulse overlap compares scan spacing with spot diameter. Negative overlap represents separated exposures.1
Calculate pulse spacing and linear overlap for laser microdissection.
When to use Microdissection Pulse Overlap
Use this tool to calculate pulse spacing and linear overlap for laser microdissection.
Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
How to use Microdissection Pulse Overlap and understand its results
- Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
- Enter stageSpeedMicrometersPerSecond, pulseRateHertz, spotDiameterMicrometers using the field table and calculation rules, then run the tool.
- Read pulseSpacingMicrometers, linearOverlapPercent. The explanation below defines the units, signs, ordering and any flags or placeholder values.
- If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.
| Input field | Meaning | Value type; see rules below | Requirement |
|---|---|---|---|
| stageSpeedMicrometersPerSecond | Stage speed (µm/s) | number; minimum: 0 | Required |
| pulseRateHertz | Pulse rate (Hz) | number; minimum: 0 | Required |
| spotDiameterMicrometers | Spot diameter (µm) | number; minimum: 0 | Required |
Enter nonnegative speed in µm/s and positive pulse rate and diameter. Return spacing in µm and signed overlap. Zero speed gives zero spacing and 100% overlap.
spacing=speed/rate; overlap%=100(1−spacing/diameter) Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.
| Output field | Meaning | Value type |
|---|---|---|
| pulseSpacingMicrometers | Pulse spacing (µm) | number; minimum: 0 |
| linearOverlapPercent | Linear pulse overlap (%) | number |
{
"input": {
"stageSpeedMicrometersPerSecond": 1000,
"pulseRateHertz": 1000,
"spotDiameterMicrometers": 2
},
"output": {
"pulseSpacingMicrometers": 1,
"linearOverlapPercent": 50
}
}Limitations and assumptions
No tissue response, thermal damage or process suitability is predicted.
Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.
Alternative or Complementary methods
Use Stealth-Dicing Pulse Spacing: Calculate stealth-dicing pulse spacing, overlap, and pulse density. For a complementary task, use Hatch Overlap: Calculate adjacent hatch overlap from effective beam diameter and hatch spacing.
References
-
Microdissection Pulse Overlap — tool.io
Similar or alternative tools
- Stealth-Dicing Pulse Spacing
Calculate stealth-dicing pulse spacing, overlap, and pulse density.
- Hatch Overlap
Calculate adjacent hatch overlap from effective beam diameter and hatch spacing.