Light-Sheet Phototoxicity
Compare light-sheet and conventional illumination dose as a phototoxicity proxy.
Description
Illumination dose compares irradiance-time products for conventional and light-sheet imaging.
Illumination dose compares irradiance-time products for conventional and light-sheet imaging. Dose reduction is a phototoxicity proxy rather than measured damage.1
Compare light-sheet and conventional illumination dose as a phototoxicity proxy.
When to use Light-Sheet Phototoxicity
Use this tool to compare light-sheet and conventional illumination dose as a phototoxicity proxy.
Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
How to use Light-Sheet Phototoxicity and understand its results
- Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
- Enter conventionalIrradianceWPerCm2, conventionalExposureSeconds, lightSheetIrradianceWPerCm2, lightSheetExposureSeconds using the field table and calculation rules, then run the tool.
- Read conventionalDoseJPerCm2, lightSheetDoseJPerCm2, doseReductionPercent. The explanation below defines the units, signs, ordering and any flags or placeholder values.
- If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.
| Input field | Meaning | Value type; see rules below | Requirement |
|---|---|---|---|
| conventionalIrradianceWPerCm2 | Conventional irradiance (W/cm²) | number; minimum: 0 | Required |
| conventionalExposureSeconds | Conventional exposure (s) | number; minimum: 0 | Required |
| lightSheetIrradianceWPerCm2 | Light-sheet irradiance (W/cm²) | number; minimum: 0 | Required |
| lightSheetExposureSeconds | Light-sheet exposure (s) | number; minimum: 0 | Required |
Enter nonnegative irradiances/times with positive conventional dose. Return both J/cm² doses and signed reduction.
dose=irradiance×time; reduction%=100(1−lightSheetDose/conventionalDose) Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.
| Output field | Meaning | Value type |
|---|---|---|
| conventionalDoseJPerCm2 | Conventional dose (J/cm²) | number; minimum: 0 |
| lightSheetDoseJPerCm2 | Light-sheet dose (J/cm²) | number; minimum: 0 |
| doseReductionPercent | Light-sheet dose reduction (%) | number |
{
"input": {
"conventionalIrradianceWPerCm2": 2,
"conventionalExposureSeconds": 10,
"lightSheetIrradianceWPerCm2": 1,
"lightSheetExposureSeconds": 10
},
"output": {
"conventionalDoseJPerCm2": 20,
"lightSheetDoseJPerCm2": 10,
"doseReductionPercent": 50
}
}Limitations and assumptions
Photochemistry, oxygen, wavelength and cellular damage are not modeled.
Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.
Alternative or Complementary methods
Use Photobiomodulation Dose: Calculate delivered energy and fluence for photobiomodulation. For a complementary task, use Two-Photon Depth Comparison: Compare measured two-photon and confocal imaging depths.
References
-
Light-Sheet Phototoxicity — tool.io
Similar or alternative tools
- Photobiomodulation Dose
Calculate delivered energy and fluence for photobiomodulation.
- Two-Photon Depth Comparison
Compare measured two-photon and confocal imaging depths.