Two-Photon Depth Comparison
Compare measured two-photon and confocal imaging depths.
Description
Imaging-depth comparison expresses measured two-photon depth relative to a confocal baseline.
Imaging-depth comparison expresses measured two-photon depth relative to a confocal baseline. It summarizes observations rather than predicting penetration.1
Compare measured two-photon and confocal imaging depths.
When to use Two-Photon Depth Comparison
Use this tool to compare measured two-photon and confocal imaging depths.
Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
How to use Two-Photon Depth Comparison and understand its results
- Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
- Enter twoPhotonDepthMillimeters, confocalDepthMillimeters using the field table and calculation rules, then run the tool.
- Read depthRatio, depthImprovementPercent. The explanation below defines the units, signs, ordering and any flags or placeholder values.
- If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.
| Input field | Meaning | Value type; see rules below | Requirement |
|---|---|---|---|
| twoPhotonDepthMillimeters | Two-photon imaging depth (mm) | number; minimum: 0 | Required |
| confocalDepthMillimeters | Confocal imaging depth (mm) | number; minimum: 0 | Required |
Enter nonnegative two-photon depth and positive confocal depth in mm. Return ratio and signed percentage improvement.
ratio=twoPhotonDepth/confocalDepth; improvement%=100(ratio−1) Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.
| Output field | Meaning | Value type |
|---|---|---|
| depthRatio | Depth ratio | number; minimum: 0 |
| depthImprovementPercent | Depth improvement (%) | number |
{
"input": {
"twoPhotonDepthMillimeters": 2,
"confocalDepthMillimeters": 0.5
},
"output": {
"depthRatio": 4,
"depthImprovementPercent": 300
}
}Limitations and assumptions
Use comparable depth definitions and imaging conditions. No tissue or resolution model is supplied.
Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.
Alternative or Complementary methods
Use STED Resolution: Estimate idealized STED resolution from depletion and saturation intensities. For a complementary task, use Confocal Lateral Resolution: Estimate confocal lateral FWHM and a simple finite-pinhole adjustment.
References
-
Two-Photon Depth Comparison — tool.io
Similar or alternative tools
- STED Resolution
Estimate idealized STED resolution from depletion and saturation intensities.
- Confocal Lateral Resolution
Estimate confocal lateral FWHM and a simple finite-pinhole adjustment.