FRET Efficiency
Calculate ideal Förster transfer efficiency from distance and Förster radius.
Description
Ideal Förster transfer efficiency depends on the sixth power of donor–acceptor separation relative to Förster radius.
Ideal Förster transfer efficiency depends on the sixth power of donor–acceptor separation relative to Förster radius. At equal distance and radius, efficiency is one half.1
Calculate ideal Förster transfer efficiency from distance and Förster radius.
When to use FRET Efficiency
Use this tool to calculate ideal Förster transfer efficiency from distance and Förster radius.
Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
How to use FRET Efficiency and understand its results
- Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
- Enter donorAcceptorDistance, forsterRadius using the field table and calculation rules, then run the tool.
- Read efficiencyFraction, efficiencyPercent. The explanation below defines the units, signs, ordering and any flags or placeholder values.
- If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.
| Input field | Meaning | Value type; see rules below | Requirement |
|---|---|---|---|
| donorAcceptorDistance | Donor–acceptor distance (length) | number; minimum: 0 | Required |
| forsterRadius | Förster radius (length) | number; minimum: 0 | Required |
Enter nonnegative distance and positive radius in matching units. Return fraction and percent.
efficiency=1/(1+(distance/R0)^6) Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.
| Output field | Meaning | Value type |
|---|---|---|
| efficiencyFraction | FRET efficiency | number; minimum: 0; maximum: 1 |
| efficiencyPercent | FRET efficiency (%) | number; minimum: 0; maximum: 100 |
{
"input": {
"donorAcceptorDistance": 5,
"forsterRadius": 5
},
"output": {
"efficiencyFraction": 0.5,
"efficiencyPercent": 50
}
}Limitations and assumptions
No orientation-factor, spectral-overlap, donor lifetime or experimental correction is derived.
Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.
Alternative or Complementary methods
Use FLIM Mean Lifetime: Calculate a count-weighted mean fluorescence lifetime from a supplied histogram. For a complementary task, use Two-Photon Depth Comparison: Compare measured two-photon and confocal imaging depths.
References
-
FRET Efficiency — tool.io
Similar or alternative tools
- FLIM Mean Lifetime
Calculate a count-weighted mean fluorescence lifetime from a supplied histogram.
- Two-Photon Depth Comparison
Compare measured two-photon and confocal imaging depths.