Description
Return sample-control difference, difference/controlSd, and sample/controlSignal.
Immune-assay data can contain raw signals, blanks, gated event counts, dilution labels, and research thresholds. Their units and denominators differ, and arithmetic on supplied values is separate from assay validation or clinical interpretation.
Return sample-control difference, difference/controlSd, and sample/controlSignal.
When to use this tool
Use Antibody Isotype Control Signal Gap when you need one transparent research-assay calculation or event-accounting check.
How to use the tool and how it works
- Enter sampleSignal, controlSignal, controlSd. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read difference, standardizedDifference, ratio. Use the output convention below.
Require positive controlSignal and controlSd. Return sample-control difference, difference/controlSd, and sample/controlSignal.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| sampleSignal | Sample Signal | Required. Finite number. |
| controlSignal | Control Signal | Required. Finite number; strictly above 0. |
| controlSd | Control Sd | Required. Finite number; strictly above 0. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| difference | Difference | Required. Finite number. |
| standardizedDifference | Standardized Difference | Required. Finite number. |
| ratio | Ratio on the numerator and denominator basis stated above. | Required. Finite number. |
{
"input": {
"sampleSignal": 150,
"controlSignal": 100,
"controlSd": 25
},
"output": {
"difference": 50,
"standardizedDifference": 2,
"ratio": 1.5
}
}Limitations and assumptions
- Signals share one scale; the supplied spread and isotype control are not evaluated for appropriateness, and no biological or diagnostic threshold is selected.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Review the complete assay protocol, calibration, controls, uncertainty, and appropriate professional interpretation.
References
Similar or alternative tools
- Serology Research Cutoff Relative Signal
Return (signal-blank)/(cutoff-blank) as relativeSignal and signal-cutoff as margin.
- Immunoassay Blank Standard Curve Residual
Subtract blank from observed, subtract predictedBlankCorrected for residual, and divide residual by positive signalSd for standardizedResidual.