Description
Return countedArea, count/countedArea, and count/sampledFraction.
Histology counting uses eligible objects, sampled areas, exclusions, and reference planes. The chosen denominator and boundary rules affect the result, while actual object identification and sampling validity require separate review.
Return countedArea, count/countedArea, and count/sampledFraction.
When to use this tool
Use Nuclear Count Field Area Normalizer when you need one supplied counting or support component under an explicit protocol.
How to use the tool and how it works
- Enter count, imagedArea, sampledFraction. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read countedArea, density, wholeFieldEquivalent. Use the output convention below.
Require nonnegative integer count, positive imagedArea, and sampledFraction in (0, 1]. Return countedArea, count/countedArea, and count/sampledFraction.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| count | Count under the stated selection and duplicate convention. | Required. Safe integer; minimum 0. |
| imagedArea | Imaged Area | Required. Finite number; strictly above 0. |
| sampledFraction | Sampled Fraction | Required. Finite number; maximum 1; strictly above 0. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| countedArea | Counted Area | Required. Finite number. |
| density | Density | Required. Finite number. |
| wholeFieldEquivalent | Whole Field Equivalent | Required. Finite number. |
{
"input": {
"count": 50,
"imagedArea": 2,
"sampledFraction": 0.5
},
"output": {
"countedArea": 1,
"density": 50,
"wholeFieldEquivalent": 100
}
}Limitations and assumptions
- CountedArea is the imaged area times the supplied sampling fraction; wholeFieldEquivalent may be fractional and does not establish representativeness or clinical cell density.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Check the full stereological or image-analysis method and appropriate professional interpretation before drawing a tissue conclusion.
References
Similar or alternative tools
- Stereology Point Count Area Fraction
Return hits/eligiblePoints, referenceArea times that fraction, and excludedPoints/(eligible+excluded).
- Platelet Research Field Area Normalizer
Return fields × area, observed per sampled area, and that rate times referenceArea.