Description
Measure a supplied developmental image's signed time-zero offset.
Developmental measurements depend on specimen identity, staging sources, coordinate registration, image scale and time-zero conventions. A section-area fraction differs from a volume, a generation-number step differs from verified lineage, and a midpoint of observations differs from an exact event time. Local scalar terms do not infer developmental mechanisms.
Measure a supplied developmental image's signed time-zero offset.
When to use this tool
Use Developmental Image Time Zero Offset when you already have imageTimestampSeconds, timeZeroTimestampSeconds and need to measure a supplied developmental image's signed time-zero offset.
How to use the tool and how it works
- Enter imageTimestampSeconds, timeZeroTimestampSeconds. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read relativeTimeSeconds. Use the output convention below.
Return imageTimestampSeconds-timeZeroTimestampSeconds.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| imageTimestampSeconds | Image Timestamp Seconds | Required. Finite number. |
| timeZeroTimestampSeconds | Time Zero Timestamp Seconds | Required. Finite number. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| relativeTimeSeconds | Relative Time Seconds | Required. Finite number. |
{
"input": {
"imageTimestampSeconds": 1600,
"timeZeroTimestampSeconds": 1000
},
"output": {
"relativeTimeSeconds": 600
}
}Limitations and assumptions
- No acquisition clock, synchronization, developmental origin or actual event time is determined.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Inspect original specimens, calibrated images, acquisition timelines and staging references for biological interpretation. Use complete image, lineage and experimental analyses when needed; local ratios, source-qualified labels and supplied coordinate arithmetic do not perform those analyses.
References
Similar or alternative tools
- Embryo Image Zstep Physical Spacing
Calculate signed physical spacing between two supplied image planes.
- Cell Tracking Division Time Midpoint
Estimate a division time as the midpoint of supplied observation bounds.