Description
Estimate a division time as the midpoint of supplied observation bounds.
Developmental measurements depend on specimen identity, staging sources, coordinate registration, image scale and time-zero conventions. A section-area fraction differs from a volume, a generation-number step differs from verified lineage, and a midpoint of observations differs from an exact event time. Local scalar terms do not infer developmental mechanisms.
Estimate a division time as the midpoint of supplied observation bounds.
When to use this tool
Use Cell Tracking Division Time Midpoint when you already have lastUndividedTime, firstDividedTime and need to estimate a division time as the midpoint of supplied observation bounds.
How to use the tool and how it works
- Enter lastUndividedTime, firstDividedTime. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read estimatedDivisionTime, uncertaintyWidth. Use the output convention below.
Require firstDividedTime>=lastUndividedTime; midpoint=last/2+first/2, uncertaintyWidth=first-last.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| lastUndividedTime | Last Undivided Time | Required. Finite number. |
| firstDividedTime | First Divided Time | Required. Finite number. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| estimatedDivisionTime | Estimated Division Time | Required. Finite number. |
| uncertaintyWidth | Full interval width between last undivided and first divided observations; not a confidence interval. | Required. Finite number. |
{
"input": {
"lastUndividedTime": 10,
"firstDividedTime": 14
},
"output": {
"estimatedDivisionTime": 12,
"uncertaintyWidth": 4
}
}Limitations and assumptions
- Width is the full observation bracket, not a standard deviation or confidence interval. No uniform event-time probability or actual division time is inferred.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Inspect original specimens, calibrated images, acquisition timelines and staging references for biological interpretation. Use complete image, lineage and experimental analyses when needed; local ratios, source-qualified labels and supplied coordinate arithmetic do not perform those analyses.
References
Similar or alternative tools
- Developmental Image Time Zero Offset
Measure a supplied developmental image's signed time-zero offset.
- Feature Quantization Single Bin Midpoint
Calculate the midpoint of supplied ordered quantization-bin edges.