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Protein Recovery Fraction Percent Tool

Express a supplied protein mass recovery on fraction and percent scales under a closed mass-balance assumption.

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Description

Divide recovered by initial for recoveryFraction and multiply by 100 for recoveryPercent.

Blank subtraction and recovery correction change a measured count's numerator before volume normalization. A negative corrected value can reveal measurement noise or an incompatible correction.

Divide recovered by initial for recoveryFraction and multiply by 100 for recoveryPercent.

When to use this tool

Use Protein Recovery Fraction Percent when you need a supplied assay recovery or volume-normalization term.

How to use the tool and how it works

  1. Enter initialProteinMass, recoveredProteinMass. Use the units and conventions below.
  2. Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
  3. Read recoveryFraction, recoveryPercent. Use the output convention below.

Require positive initialProteinMass and recovered mass within it. Divide recovered by initial for recoveryFraction and multiply by 100 for recoveryPercent.1

Input fieldMeaning and unitsAccepted input
initialProteinMassInitial Protein MassRequired. Finite number; strictly above 0.
recoveredProteinMassRecovered Protein MassRequired. Finite number; minimum 0.
Output fieldInterpretationReturned shape
recoveryFractionRecovery FractionRequired. Finite number.
recoveryPercentRecovery PercentRequired. Finite number.
json
{
  "input": {
    "initialProteinMass": 80,
    "recoveredProteinMass": 60
  },
  "output": {
    "recoveryFraction": 0.75,
    "recoveryPercent": 75
  }
}

Limitations and assumptions

  • Use matching mass units; identity, purity, biological activity, method validity, and causes of loss are not determined.
  • Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
  • Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.

Alternative or Complementary methods

Review blank and recovery measurements and use a validated assay-specific analysis for scientific interpretation.

References

  1. Calculation and input contract: biotechnology/protein-recovery-fraction-percent — tool.io

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