Skip to content

Main content

Single Particle Localization Error Radius

Combine signed 2D localization-coordinate errors into a Euclidean error radius without estimating uncertainty from images.

Loading tool…

Description

Calculate a supplied two-axis localization-error magnitude.

Biophysical scalar models require explicit signal, concentration, temperature, energy and geometric conventions. Effective concentration differs from dimensionless activity, a single displacement differs from mean-square displacement, and a fluorescence yield ratio needs absorption and refractive-index corrections. Supplied model parameters do not establish that the model applies to an experiment.

Calculate a supplied two-axis localization-error magnitude.

When to use this tool

Use Single Particle Localization Error Radius when you already have xError, yError and need to calculate a supplied two-axis localization-error magnitude.

How to use the tool and how it works

  1. Enter xError, yError. Use the units and conventions below.
  2. Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
  3. Read radialError. Use the output convention below.

Return Math.hypot(xError,yError).1

Input fieldMeaning and unitsAccepted input
xErrorX ErrorRequired. Finite number.
yErrorY ErrorRequired. Finite number.
Output fieldInterpretationReturned shape
radialErrorRadial ErrorRequired. Finite number.
json
{
  "input": {
    "xError": 3,
    "yError": 4
  },
  "output": {
    "radialError": 5
  }
}

Limitations and assumptions

  • The output uses the matching input length unit. No error distribution, confidence radius, image localization, calibration or actual position is estimated.
  • Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
  • Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.

Alternative or Complementary methods

Use the complete physical model, calibrated measurements and uncertainty analysis. Check reference states, photon-signal bases, diffusion assumptions and binding-constant conventions before interpreting local contributions as physical measurements.

References

  1. Calculation and input contract: biophysics/single-particle-localization-error-radius — tool.io

Similar or alternative tools