Description
Preserve a supplied microscopy PSF radius and its explicit definition.
Biophysical scalar models require explicit signal, concentration, temperature, energy and geometric conventions. Effective concentration differs from dimensionless activity, a single displacement differs from mean-square displacement, and a fluorescence yield ratio needs absorption and refractive-index corrections. Supplied model parameters do not establish that the model applies to an experiment.
Preserve a supplied microscopy PSF radius and its explicit definition.
When to use this tool
Use Microscopy PSF Radius Reference when you already have imageId, radiusText, radiusDefinition and need to preserve a supplied microscopy PSF radius and its explicit definition.
How to use the tool and how it works
- Enter imageId, radiusText, radiusDefinition. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read token. Use the output convention below.
Enter imageId, radiusText, radiusDefinition using the field constraints below. Encode these values, in exactly that order, as a JSON array and return the serialized array as text in token. String values are preserved without trimming or external terminology lookup.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| imageId | Image Id | Required. Text; minimum UTF-16 length 1; maximum UTF-16 length 8192. |
| radiusText | Radius Text | Required. Text; minimum UTF-16 length 1; maximum UTF-16 length 8192. |
| radiusDefinition | Radius Definition | Required. Text; minimum UTF-16 length 1; maximum UTF-16 length 8192. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| token | Token | Required. Text; maximum UTF-16 length 8192. |
{
"input": {
"imageId": "I17",
"radiusText": "250 nm",
"radiusDefinition": "first minimum"
},
"output": {
"token": "[\"I17\",\"250 nm\",\"first minimum\"]"
}
}Limitations and assumptions
- The result records supplied values without equating different resolution conventions. Sources and asserted relationships are not independently authenticated.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
Alternative or Complementary methods
Use the complete physical model, calibrated measurements and uncertainty analysis. Check reference states, photon-signal bases, diffusion assumptions and binding-constant conventions before interpreting local contributions as physical measurements.
References
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