Description
Return blank-corrected donor/acceptor signals and acceptor fraction.
Fluorescence signals combine excitation, emission, detector response and background. An intensity ratio, effective lifetime or anisotropy correction describes a supplied optical model; measured signal fractions and ideal energy-transfer efficiencies are different quantities and need appropriate calibration.
Return blank-corrected donor/acceptor signals and acceptor fraction.
When to use this tool
Use Biophysics: FRET Backgroundsubtracted Ratio when checking the stated fluorescence, lifetime, transfer or polarization correction.
How to use the tool and how it works
- Enter donor, acceptor, donorBackground, acceptorBackground. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read result. Expand result to inspect the returned fields.
Return blank-corrected donor/acceptor signals and acceptor fraction. Require both corrected signals nonnegative and their sum positive; acceptorFraction = correctedAcceptor/(correctedAcceptor+correctedDonor).1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| donor | Donor | Required. Finite number. |
| acceptor | Acceptor | Required. Finite number. |
| donorBackground | Donor Background | Required. Finite number. |
| acceptorBackground | Acceptor Background | Required. Finite number. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| result | Returned calculated value or nested object under the stated output convention. | Required. Object. |
| result.correctedDonor | Corrected Donor | Calculated result member: number. |
| result.correctedAcceptor | Corrected Acceptor | Calculated result member: number. |
| result.acceptorFraction | Corrected acceptor/(corrected donor+corrected acceptor); not automatically a corrected FRET efficiency. | Calculated result member: number. |
{
"input": {
"donor": 10,
"acceptor": 8,
"donorBackground": 2,
"acceptorBackground": 4
},
"output": {
"result": {
"correctedDonor": 8,
"correctedAcceptor": 4,
"acceptorFraction": 0.3333333333333333
}
}
}Limitations and assumptions
- This simple intensity fraction is not a fully corrected FRET efficiency or distance; leakage and detector response are not modeled.
- Outputs are nested inside result. Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use calibrated optical measurements and a complete suitable photophysical model, including background, cross-talk and detector effects, for molecular or kinetic inference.
References
Similar or alternative tools
- FRET Distance Efficiency Inverse Term
Invert the supplied ideal FRET efficiency relation.
- Fluorescence Donor Leakage Term Tool
Subtract an explicitly calibrated donor leakage term without full FRET correction or efficiency inference.