Description
Calculate a supplied relative fluorescence quantum-yield term.
Biophysical scalar models require explicit signal, concentration, temperature, energy and geometric conventions. Effective concentration differs from dimensionless activity, a single displacement differs from mean-square displacement, and a fluorescence yield ratio needs absorption and refractive-index corrections. Supplied model parameters do not establish that the model applies to an experiment.
Calculate a supplied relative fluorescence quantum-yield term.
When to use this tool
Use Fluorescence Quantum Yield Reference Ratio when you already have sampleIntegratedSignal, referenceIntegratedSignal, sampleAbsorbance, referenceAbsorbance, referenceYield, sampleRefractiveIndex, referenceRefractiveIndex and need to calculate a supplied relative fluorescence quantum-yield term.
How to use the tool and how it works
- Enter sampleIntegratedSignal, referenceIntegratedSignal, sampleAbsorbance, referenceAbsorbance, referenceYield, sampleRefractiveIndex, referenceRefractiveIndex. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read relativeYield. Use the output convention below.
Require nonnegative sample signal, reference yield in [0,1] and positive reference signal, absorbances and refractive indices. Multiply reference yield by signal ratio, absorbed-reference/absorbed-sample fraction ratio using -expm1(-ln(10)*A), and (nSample/nReference)^2.1
Relative fluorescence quantum-yield comparison uses integrated photon signals, absorbed excitation fractions and a squared refractive-index ratio. Comparable excitation, calibrated spectral response and suitable sample/reference conditions remain experimental requirements.2
| Input field | Meaning and units | Accepted input |
|---|---|---|
| sampleIntegratedSignal | Integrated fluorescence signal on a consistent photon-number basis with the reference. | Required. Finite number; minimum 0. |
| referenceIntegratedSignal | Reference Integrated Signal | Required. Finite number; strictly above 0. |
| sampleAbsorbance | Positive dimensionless base-10 absorbance at excitation, not an absorption fraction. | Required. Finite number; strictly above 0. |
| referenceAbsorbance | Reference Absorbance | Required. Finite number; strictly above 0. |
| referenceYield | Reference quantum yield in [0,1] supplied under comparable conditions. | Required. Finite number; minimum 0; maximum 1. |
| sampleRefractiveIndex | Sample Refractive Index | Required. Finite number; strictly above 0. |
| referenceRefractiveIndex | Reference Refractive Index | Required. Finite number; strictly above 0. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| relativeYield | Reference yield times signal ratio, absorbed-fraction correction and (n_sample/n_reference)²; not clamped to one. | Required. Finite number. |
{
"input": {
"sampleIntegratedSignal": 80,
"referenceIntegratedSignal": 100,
"sampleAbsorbance": 0.1,
"referenceAbsorbance": 0.1,
"referenceYield": 0.5,
"sampleRefractiveIndex": 1.33,
"referenceRefractiveIndex": 1.33
},
"output": {
"relativeYield": 0.4
}
}Limitations and assumptions
- Absorbance is base-10 optical absorbance. Compatible integration, reference and geometry assumptions are supplied; no spectra, response corrections or valid yield is established. Output is not clamped to [0,1].
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use the complete physical model, calibrated measurements and uncertainty analysis. Check reference states, photon-signal bases, diffusion assumptions and binding-constant conventions before interpreting local contributions as physical measurements.
References
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