Use Steady-State Concentration when you need to predict the average plasma concentration at steady state as the infusion rate times bioavailability divided by clearance. Enter Dose rate, Clearance, and Bioavailability fraction using the definitions and units below so the result can be reproduced.

Dose rate

Dosing rate in milligrams per hour; must be greater than zero.

Clearance

Clearance in litres per hour; must be greater than zero.

Bioavailability fraction

Systemic bioavailability fraction between zero and one; defaults to one.

The published tool contract uses this rule: Predict the average plasma concentration at steady state as the infusion rate times bioavailability divided by clearance.

Steady-state concentration

Average concentration at steady state in mg/L.

A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small. Bioavailability fraction must remain between 0 and 1.

Retain the original Dose rate, Clearance, and Bioavailability fraction values and their units alongside the result. Primer Concentration nM is useful when you instead need to convert a single-stranded DNA primer mass concentration in ng per microlitre to nanomolar using an approximate average nucleotide mass of 330 grams per mole. Cell Concentration From Dilution provides another view by helping you recover the original cell concentration by scaling a counted dilution.

• Primer Concentration nM

  Convert a single-stranded DNA primer mass concentration in ng per microlitre to nanomolar using an approximate average nucleotide mass of 330 grams per mole.

• Cell Concentration From Dilution

  Recover the original cell concentration by scaling a counted dilution.

• DNA Conc From A260

  Convert an A260 absorbance reading into double-stranded DNA concentration using the 50 ng/µL per absorbance unit factor.