Use RIN Proxy when you need to estimate an RNA integrity proxy on a one to ten scale from the ratio of 28S to total rRNA peak area. Enter 28S peak area and 18S peak area using the definitions and units below so the result can be reproduced.

28S peak area

A finite measured or assumed value.

18S peak area

A finite measured or assumed value.

The published tool contract uses this rule: Estimate an RNA integrity proxy on a one to ten scale from the ratio of 28S to total rRNA peak area.

Integrity proxy

The calculated integrity proxy.

The output is an estimate tied to the stated model and coefficients; it is not a direct measurement and should retain that label when reported.

Retain the original 28S peak area and 18S peak area values and their units alongside the result. RNA Copy Number is useful when you instead need to calculate the number of RNA molecules from mass in nanograms and length in nucleotides. Plasmid Copies Per Cell provides another view by helping you estimate plasmid copies per cell from plasmid and genomic DNA masses, correcting the mass ratio for their different molecule lengths.

• RNA Copy Number

  Calculate the number of RNA molecules from mass in nanograms and length in nucleotides.

• Plasmid Copies Per Cell

  Estimate plasmid copies per cell from plasmid and genomic DNA masses, correcting the mass ratio for their different molecule lengths.

• RNA Conc From A260

  Convert an A260 absorbance reading into RNA concentration using the 40 ng/µL per absorbance unit factor.