Use Plasmid Copies Per Cell when you need to estimate plasmid copies per cell from plasmid and genomic DNA masses, correcting the mass ratio for their different molecule lengths. Enter Plasmid mass, Genome mass, Plasmid length, Genome length, and Host genome copies using the definitions and units below so the result can be reproduced.

Plasmid mass

A finite measured or assumed value, expressed in ng.

Genome mass

A finite measured or assumed value, expressed in ng.

Plasmid length

An integer count or setting greater than or equal to 1, expressed in bp.

Genome length

An integer count or setting greater than or equal to 1, expressed in bp.

Host genome copies

An integer count or setting greater than or equal to 1.

The published tool contract uses this rule: Estimate plasmid copies per cell from plasmid and genomic DNA masses, correcting the mass ratio for their different molecule lengths.^[1]

Plasmid copies per cell

The calculated plasmid copies per cell.

The output is an estimate tied to the stated model and coefficients; it is not a direct measurement and should retain that label when reported.

Retain the original Plasmid mass, Genome mass, Plasmid length, Genome length, and Host genome copies values and their units alongside the result. Plasmid Copy Number is useful when you instead need to estimate plasmid copies per genome as the concentration ratio scaled by the inverse ratio of molecule lengths. qPCR Copy Number provides another view by helping you convert a double-stranded DNA mass in nanograms to molecule count using length and the mean base pair mass of 650 daltons.

• Plasmid Copy Number

  Estimate plasmid copies per genome as the concentration ratio scaled by the inverse ratio of molecule lengths.

• qPCR Copy Number

  Convert a double-stranded DNA mass in nanograms to molecule count using length and the mean base pair mass of 650 daltons.

• DNA Copy Number

  Calculate the number of DNA molecules from mass in nanograms and length in base pairs.