Use Primer Efficiency From Slope when you need to derive qPCR primer efficiency in percent from the standard curve slope. Enter Standard curve slope using the definitions and units below so the result can be reproduced.

Standard curve slope

A finite measured or assumed value.

The published tool contract uses this rule: Derive qPCR primer efficiency in percent from the standard curve slope.^[1]

Primer efficiency

The calculated primer efficiency in %.

The calculation does not propagate measurement uncertainty, sampling error, or biological and chemical variability.

Retain the original Standard curve slope values and their units alongside the result. 3' GC Clamp Count is useful when you instead need to count guanine and cytosine bases among the last three nucleotides of a primer sequence. Primer Concentration nM provides another view by helping you convert a single-stranded DNA primer mass concentration in ng per microlitre to nanomolar using an approximate average nucleotide mass of 330 grams per mole.

• 3' GC Clamp Count

  Count guanine and cytosine bases among the last three nucleotides of a primer sequence.

• Primer Concentration nM

  Convert a single-stranded DNA primer mass concentration in ng per microlitre to nanomolar using an approximate average nucleotide mass of 330 grams per mole.

• Primer GC Percent

  Calculate the GC percentage of a primer sequence for clamp design checks.