3' GC Clamp Count
Count guanine and cytosine bases among the last three nucleotides of a primer sequence.
Description
Count guanine and cytosine bases among the last three nucleotides of a primer sequence.
3' GC Clamp Count is a focused biology tool that count guanine and cytosine bases among the last three nucleotides of a primer sequence. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use 3' GC Clamp Count
Use 3' GC Clamp Count when you need to count guanine and cytosine bases among the last three nucleotides of a primer sequence. Enter Sequence using the definitions and units below so the result can be reproduced.
- Sequence
- A sequence, category, or text value.
How 3' GC Clamp Count calculates the result
The published tool contract uses this rule: Count guanine and cytosine bases among the last three nucleotides of a primer sequence.
- GC clamp count
- The calculated gc clamp count.
Limitations of 3' GC Clamp Count
The calculation does not propagate measurement uncertainty, sampling error, or biological and chemical variability.
Alternative or Complementary measurements
Retain the original Sequence values and their units alongside the result. Primer GC Percent is useful when you instead need to calculate the GC percentage of a primer sequence for clamp design checks. GC Clamp Check provides another view by helping you check the GC clamp of a primer by counting guanine and cytosine bases among the last five nucleotides.
References
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Primer (molecular biology) — Wikipedia contributors
Similar or alternative tools
- Primer GC Percent
Calculate the GC percentage of a primer sequence for clamp design checks.
- GC Clamp Check
Check the GC clamp of a primer by counting guanine and cytosine bases among the last five nucleotides.
- Hairpin Tm Estimate
Estimate primer hairpin stability by searching the sequence against its own reverse complement for the longest internal inverted-repeat stem of three to eight non-overlapping bases and applying the Wallace rule of four degrees per GC pair and two per AT pair.