Use Oligo Extinction Coefficient when you need to estimate the molar extinction coefficient of an oligonucleotide from base composition using nearest-neighbor-less base constants. Enter Sequence using the definitions and units below so the result can be reproduced.

Sequence

A sequence, category, or text value.

The published tool contract uses this rule: Estimate the molar extinction coefficient of an oligonucleotide from base composition using nearest-neighbor-less base constants.^[1]

Extinction coefficient

The calculated extinction coefficient.

The output is an estimate tied to the stated model and coefficients; it is not a direct measurement and should retain that label when reported.

Retain the original Sequence values and their units alongside the result. Oligo MW Approx is useful when you instead need to approximate single-stranded oligonucleotide molecular weight as nucleotide length times the average nucleotide mass of 330 grams per mole. Pedigree F Accumulated provides another view by helping you accumulate an inbreeding coefficient as the base coefficient plus the remaining relationship budget times one path contribution.

• Oligo MW Approx

  Approximate single-stranded oligonucleotide molecular weight as nucleotide length times the average nucleotide mass of 330 grams per mole.

• Pedigree F Accumulated

  Accumulate an inbreeding coefficient as the base coefficient plus the remaining relationship budget times one path contribution.

• Coancestry Coefficient

  Compute the coancestry or kinship phi of two individuals as half of their additive relationship coefficient.