Use Oligo MW Approx when you need to approximate single-stranded oligonucleotide molecular weight as nucleotide length times the average nucleotide mass of 330 grams per mole. Enter Length nt using the definitions and units below so the result can be reproduced.

Length nt

An integer count or setting greater than or equal to 1.

The published tool contract uses this rule: Approximate single-stranded oligonucleotide molecular weight as nucleotide length times the average nucleotide mass of 330 grams per mole.^[1]

Oligo molecular weight Da

The calculated oligo molecular weight da in Da.

The calculation does not propagate measurement uncertainty, sampling error, or biological and chemical variability.

Retain the original Length nt values and their units alongside the result. Protein MW From Length is useful when you instead need to estimate protein molecular weight from residue count using the mean residue mass of 110 daltons. Oligo Extinction Coefficient provides another view by helping you estimate the molar extinction coefficient of an oligonucleotide from base composition using nearest-neighbor-less base constants.

• Protein MW From Length

  Estimate protein molecular weight from residue count using the mean residue mass of 110 daltons.

• Oligo Extinction Coefficient

  Estimate the molar extinction coefficient of an oligonucleotide from base composition using nearest-neighbor-less base constants.

• Nearest Neighbor Tm Approx

  Approximate primer melting temperature with a salt-adjusted nearest-neighbor style formula.