GC Rule Tm
Estimate DNA melting temperature with the GC rule for sequences longer than 13 bases.
Description
Estimate DNA melting temperature with the GC rule for sequences longer than 13 bases.
GC Rule Tm is a focused biology tool that estimate DNA melting temperature with the GC rule for sequences longer than 13 bases. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use GC Rule Tm
Use GC Rule Tm when you need to estimate DNA melting temperature with the GC rule for sequences longer than 13 bases. Enter Sequence using the definitions and units below so the result can be reproduced.
- Sequence
- A sequence, category, or text value.
How GC Rule Tm calculates the result
The published tool contract uses this rule: Estimate DNA melting temperature with the GC rule for sequences longer than 13 bases.1
- Melting temperature
- The calculated melting temperature in °C.
Limitations of GC Rule Tm
The output is an estimate tied to the stated model and coefficients; it is not a direct measurement and should retain that label when reported.
Alternative or Complementary measurements
Retain the original Sequence values and their units alongside the result. Wallace Tm is useful when you instead need to estimate DNA melting temperature with the Wallace rule for sequences up to 14 bases. Salt-Adjusted Tm provides another view by helping you estimate DNA melting temperature with a monovalent salt correction from GC fraction and sequence length.
References
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Nucleic acid thermodynamics — Wikipedia contributors
Similar or alternative tools
- Wallace Tm
Estimate DNA melting temperature with the Wallace rule for sequences up to 14 bases.
- Salt-Adjusted Tm
Estimate DNA melting temperature with a monovalent salt correction from GC fraction and sequence length.
- Nearest Neighbor Tm Approx
Approximate primer melting temperature with a salt-adjusted nearest-neighbor style formula.