Use Salt-Adjusted Tm when you need to estimate DNA melting temperature with a monovalent salt correction from GC fraction and sequence length. Enter GC fraction, Sequence length, and Monovalent salt concentration using the definitions and units below so the result can be reproduced.

GC fraction

A finite measured or assumed value from 0 through 1.

Sequence length

An integer count or setting, expressed in bp.

Monovalent salt concentration

A finite measured or assumed value, expressed in M.

The published tool contract uses this rule: Estimate DNA melting temperature with a monovalent salt correction from GC fraction and sequence length.

Melting temperature

The calculated melting temperature in °C.

The output is an estimate tied to the stated model and coefficients; it is not a direct measurement and should retain that label when reported. GC fraction must remain between 0 and 1.

Retain the original GC fraction, Sequence length, and Monovalent salt concentration values and their units alongside the result. GC Rule Tm is useful when you instead need to estimate DNA melting temperature with the GC rule for sequences longer than 13 bases. Wallace Tm provides another view by helping you estimate DNA melting temperature with the Wallace rule for sequences up to 14 bases.

• GC Rule Tm

  Estimate DNA melting temperature with the GC rule for sequences longer than 13 bases.

• Wallace Tm

  Estimate DNA melting temperature with the Wallace rule for sequences up to 14 bases.

• Nearest Neighbor Tm Approx

  Approximate primer melting temperature with a salt-adjusted nearest-neighbor style formula.