IC50 Inhibition Conc
Estimate the half-maximal inhibitory concentration by inverting the Hill equation.
Description
Estimate the half-maximal inhibitory concentration by inverting the Hill equation.
IC50 Inhibition Conc is a focused biology tool that estimate the half-maximal inhibitory concentration by inverting the Hill equation. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use IC50 Inhibition Conc
Use IC50 Inhibition Conc when you need to estimate the half-maximal inhibitory concentration by inverting the Hill equation. Enter Concentration, Inhibition fraction, and Slope factor using the definitions and units below so the result can be reproduced.
- Concentration
- A finite measured or assumed value.
- Inhibition fraction
- A finite measured or assumed value.
- Slope factor
- A finite measured or assumed value.
How IC50 Inhibition Conc calculates the result
The published tool contract uses this rule: Estimate the half-maximal inhibitory concentration by inverting the Hill equation.
- IC50 concentration
- The calculated ic50 concentration.
Limitations of IC50 Inhibition Conc
The output is an estimate tied to the stated model and coefficients; it is not a direct measurement and should retain that label when reported.
Alternative or Complementary measurements
Retain the original Concentration, Inhibition fraction, and Slope factor values and their units alongside the result. Biological Half-Life is useful when you instead need to compute the elimination half-life from volume of distribution and clearance. Cheng-Prusoff IC50 provides another view by helping you convert an observed IC50 from a competitive binding assay to Ki with the Cheng-Prusoff equation: Ki = IC50 / (1 + [L]/Kd). The relationship assumes equilibrium competition at a single binding site without substantial tracer depletion.
References
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IC50 — Wikipedia contributors
Similar or alternative tools
- Biological Half-Life
Compute the elimination half-life from volume of distribution and clearance.
- Cheng-Prusoff IC50
Convert an observed IC50 from a competitive binding assay to Ki with the Cheng-Prusoff equation: Ki = IC50 / (1 + [L]/Kd). The relationship assumes equilibrium competition at a single binding site without substantial tracer depletion.
- Bradford Protein Conc
Convert a Bradford assay A595 reading into protein concentration using the slope of the standard curve.