Cheng-Prusoff IC50
Convert an observed IC50 from a competitive binding assay to Ki with the Cheng-Prusoff equation: Ki = IC50 / (1 + [L]/Kd). The relationship assumes equilibrium competition at a single binding site without substantial tracer depletion.
Description
Convert an observed IC50 from a competitive binding assay to Ki with the Cheng-Prusoff equation: Ki = IC50 / (1 + [L]/Kd). The relationship assumes equilibrium competition at a single binding site without substantial tracer depletion.