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Ligand Binding Total Free Balance

Subtract supplied free from total ligand on a shared concentration basis, preserving inconsistent negative balances.

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Description

Calculate supplied total-minus-free ligand amount.

Biochemical quantities depend on species definitions, mass versus amount basis, reference states, blank models and assay conditions. A free/bound difference does not establish equilibrium, a per-volume activity differs from activity per protein mass, and a dilution factor differs from its reciprocal aliquot fraction.

Calculate supplied total-minus-free ligand amount.

When to use this tool

Use Ligand Binding Total Free Balance when you already have totalLigand, freeLigand and need to calculate supplied total-minus-free ligand amount.

How to use the tool and how it works

  1. Enter totalLigand, freeLigand. Use the units and conventions below.
  2. Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
  3. Read boundLigand. Use the output convention below.

Require both nonnegative; return totalLigand-freeLigand.1

Input fieldMeaning and unitsAccepted input
totalLigandTotal LigandRequired. Finite number; minimum 0.
freeLigandFree LigandRequired. Finite number; minimum 0.
Output fieldInterpretationReturned shape
boundLigandtotalLigand-freeLigand; negative discrepancies are accepted rather than a binding equilibrium being solved.Required. Finite number.
json
{
  "input": {
    "totalLigand": 12,
    "freeLigand": 5
  },
  "output": {
    "boundLigand": 7
  }
}

Limitations and assumptions

  • Negative discrepancies are retained. Matching units and chemical definitions are assumed; no binding model, equilibrium, stoichiometry or physical consistency is verified.
  • Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
  • Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.

Alternative or Complementary methods

Use the full assay, reaction and calibration method with verified sample records. Check units, reference definitions, blanks and experimental assumptions before interpreting a local product, fraction or rate as a valid biochemical measurement.

References

  1. Calculation and input contract: biochemistry/ligand-binding-total-free-balance — tool.io

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