Description
Calculate supplied free and bound fractions of a total amount.
Biochemical quantities depend on species definitions, mass versus amount basis, reference states, blank models and assay conditions. A free/bound difference does not establish equilibrium, a per-volume activity differs from activity per protein mass, and a dilution factor differs from its reciprocal aliquot fraction.
Calculate supplied free and bound fractions of a total amount.
When to use this tool
Use Binding Fraction Free Bound Crosswalk when you already have freeAmount, boundAmount and need to calculate supplied free and bound fractions of a total amount.
How to use the tool and how it works
- Enter freeAmount, boundAmount. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read boundFraction, freeFraction, totalAmount. Use the output convention below.
Require free/bound amounts>=0 and their sum>0; return both amount/sum fractions and the sum.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| freeAmount | Free Amount | Required. Finite number; minimum 0. |
| boundAmount | Bound Amount | Required. Finite number; minimum 0. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| boundFraction | Bound Fraction | Required. Finite number. |
| freeFraction | Free Fraction | Required. Finite number. |
| totalAmount | Total Amount | Required. Finite number. |
{
"input": {
"freeAmount": 20,
"boundAmount": 80
},
"output": {
"boundFraction": 0.8,
"freeFraction": 0.2,
"totalAmount": 100
}
}Limitations and assumptions
- Use the same unit and species scope. Nonfinite returned totals are rejected; no binding equilibrium, fractionation, affinity or physical consistency is inferred.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use the full assay, reaction and calibration method with verified sample records. Check units, reference definitions, blanks and experimental assumptions before interpreting a local product, fraction or rate as a valid biochemical measurement.
References
Similar or alternative tools
- Ligand Binding Total Free Balance
Calculate supplied total-minus-free ligand amount.
- Protein Fractional Labeling Input
Calculate a supplied labeled-protein fraction.