Of Open Sealed Reaction

Decode paired open/sealed oxidative-fermentative observations while separating no-growth and discordant cases.

Description

Open and sealed oxidative-fermentative tube observations can distinguish acid patterns under different oxygen conditions.

Open and sealed oxidative-fermentative tube observations can distinguish acid patterns under different oxygen conditions. No growth and discordant sealed-only acid deserve their own outcomes.^[1]

Decode paired open/sealed oxidative-fermentative observations while separating no-growth and discordant cases.

When to use Of Open Sealed Reaction

Use this tool to decode paired open/sealed oxidative-fermentative observations while separating no-growth and discordant cases.

Prepare the sample, count, growth or assay measurements requested by this tool. Confirm units, sampling periods and reporting bases; supply independently established comparison criteria where requested.

How to use Of Open Sealed Reaction and understand its results

Prepare the sample, count, growth or assay measurements requested by this tool. Confirm units, sampling periods and reporting bases; supply independently established comparison criteria where requested.
Enter openTubeAcid, sealedTubeAcid, openGrowth, sealedGrowth using the field table and calculation rules, then run the tool.
Read metabolismPattern. The explanation below defines the units, signs, ordering and any flags or placeholder values.
If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.

Input field	Meaning	Value type; see rules below	Requirement
openTubeAcid	Open tube acid	boolean	Required
sealedTubeAcid	Sealed tube acid	boolean	Required
openGrowth	Open growth	boolean	Required
sealedGrowth	Sealed growth	boolean	Required

Enter growth and acid flags for both tubes. An acid flag without growth in the corresponding tube is an input error. The tool returns one metabolismPattern: fermentative, oxidative, discordant-sealed-only, no-acid-observed when some growth occurs without acid, or uninterpretable-no-growth when neither grows.

both acid → fermentative; open-only → oxidative; sealed-only → discordant-sealed-only

Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.

Output field	Meaning	Value type
metabolismPattern	Metabolism pattern	string

json

{
  "input": {
    "openTubeAcid": true,
    "sealedTubeAcid": false,
    "openGrowth": true,
    "sealedGrowth": true
  },
  "output": {
    "metabolismPattern": "oxidative"
  }
}

Limitations and assumptions

This decodes observations without identifying an organism or checking controls and incubation conditions. A pattern is not a complete metabolic characterization and no assay procedure is generated.

Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.

Alternative or Complementary methods

Use TSI Slant Butt Logic: Interpret valid TSI reaction pairs with acid-butt inference under sulfide blackening; not a species identification. For a complementary task, use Minimal Medium Rescue Screen: Screen paired minimal, complete, and supplemented-medium observations for a growth-rescue pattern; does not identify the deficient pathway.

References

Of Open Sealed Reaction — tool.io

Similar or alternative tools

TSI Slant Butt Logic
Interpret valid TSI reaction pairs with acid-butt inference under sulfide blackening; not a species identification.
Minimal Medium Rescue Screen
Screen paired minimal, complete, and supplemented-medium observations for a growth-rescue pattern; does not identify the deficient pathway.